The isolation and regeneration of potato (Solanum tuberosum L) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid . The Isolation of regeneratiom of potato (Solanum tuberosum L.) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid. One of new technologies in order to create hybrid plant from different species is protoplast fusion. This technique involves protoplast isolation, purification.

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Journal on Agriculture Science. Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test.

Agriculture General Country of publisher: Isolasi dan fusi protoplas dari kedelai, buncis, dan tembakau. Publication Year Publication Year. Journal Homepage Journal Title: Protoplast were collected by centrifugation of g and a thin layer of the suspension was irradiated with ultraviolet light. Protoplast were cultured on the medium of Ikeda and Uchimia.

Callus wich has been two times resubcultured was suspended in the digestive medium without driselase, CaCl2, and MES and incubated in lowlight intensity by constant shaking at rpm in reciprocal water shaker at 30 celcius centigrade. Results 1 – 1 of 1.


Close Go to Workspace. Cell wall generation was observed. The highest protoplast density and number of viable protoplasts achieved in the combined treatment of 0.

Indonesian, English Full-text formats available: Reference Number Reference Number. Funding for sustainable Open Access incl.

Prosiding Seminar Nasional Biologi

Looks like Javascript is disabled on your browser. The first factor was concentrations of sucrose which consists of three levels namely 0. Indonesia Language of fulltext: Standard Search Advanced Search. Isolation of protoplast from soybean, cowpea, and tobacco and their fusion.

Please provide a name for this query: The second factor was incubation time which consists of four levels namely 2 hours, 4 hours, 6 hours and 8 hours.

Isolasi Protoplas Tanaman Kacang Panjang Secara Enzimatis – Neliti

Leaf protoplast were releasaed isolsai h, soybean and tobacco callus protoplast in h, and cowpeas callus protoplast in h of incubation. Javascript must be enabled for narrowing. The results showed that there was effect of treatment for all variables observed. They were incibated in full darkness at 27 Celcius centigrade by constant shaking at 50 rpm orbitor shaker.

Isolation of protoplast from soybean, cowpea, and tobacco and..|INIS

The factorial experiment consisted of two factors that was arranged by completely randomized design CRD with ten replications. Our members Our publisher members Our iwolasi Our volunteers. On both medium leaf protoplast, irradiated protoplasts and their fused do not regenerate cell wall and all cultured died out within four weeks incubation.


The obyectives of the research was to investigate the effect of combination treatment of sucrose concentrations and incubation time on protoplast isolation of the leaves mesophyll orchid of Paraphalaenopsis layockii.

Fusion was induced with PEG solution according to Uchimia and fused protoplasts were collected isklasi centrifugation of g. For soybean leaf, the medium was enriched with driselase and pectolyase Y Regeneration of cell wall observed progessively in mother protoplast from tobacco, cowpea A27, E1, and H6 and fused protoplast of soybean with tobacco, tobacco with cowpea C4, E1, and H6soybean with cowpea C4 and isolasj cowpea C4 and cowpea E1.

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