Nutr Cancer. ;68(1) doi: / Epub Jan Inula Viscosa Extracts Induces Telomere Shortening and Apoptosis . Overview of the medicinal plant Inula viscosa, Inula viscosa R&D, cultivation, processing, extraction and formulations by Avisco Ltd. Traditional medicine uses of Inula viscosa, folk medicine practices and refernces from traditional medicine books.

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Little-differentiated callus cells are synthetically active and produce, as do its leaf cells, numerous polar compounds that could be of pharmaceutical interest. All palisade-parenchyma cells possess vacuolar inclusions. Our observations revealed that tissue cultures actually produce anti-fungal factors not found in the leaves or not released from them because the yeasts, in infected cultures, attack the leaves, which deteriorate before producing calluses.

General information is given in Table 1. Crystals are common blue arrows in Fig. This presupposes that callus cells or cell suspension cultures are able to produce active secondary compounds in the same way as are differentiated cells Zenk,and from which an additional advantage becomes evident. Calluses produced from leaf-cell cultures were also subjected to histochemical reagents and tested with GC-MS and TLC to investigate their secreting ability compared with that in leaves.

It seems that although most histochemical reactions are positive for both leaves and calluses, the volatile constituents of the leaves are not present in the callus cells.

An interesting observation on developed callus masses is related to the well-known phenomenon of the clear zone appearing round the colonies of antibiotic-producing bacteria or fungi when they are approached by expanding colonies of other susceptible micro-organisms. Inuoa Wikipedia, the free encyclopedia. In de-osmicated sections from plastic embedded tissue they appear brownish in colour and seem to be trapped among the grey-coloured phenolic inual of the vacuole white arrows in Fig.

Control of Plant Diseases by Extracts of Inula viscosa.

Stomatal complexes of the anomocytic type Figs 2, 3lying above well-developed substomatal chambers of the mesophyll white arrow in Fig. One species among these is Inula viscosa L. Free hand sections of fresh leaf tissue ft were stained with traditional reagents: Their cells reacted positively for the same compounds as the primary calluses and produced equally intense staining. Fungistatic activity of a sequiterpene lactone tomentosin isolated from fresh Inula viscosa Asteraceae flowers from the Pulgia region.


Histochemical investigations revealed that various substance groups were localized in the leaf. Developmental evidence helps resolve the evolutionary origins of visdosa appendages in Globba Zingiberaceae. Flavonoids of Inula brittanica protect cultured cortical cells from necrotic cell death induced by glutamate. Dittrichia viscosa Scientific classification Kingdom: Protective hairs, mainly on the central nerve. Some of the compounds investigated in the fresh leaf and all the compounds of callus extracts of Inula viscosa ihula GC-MS.

Control of Plant Diseases by Extracts of Inula viscosa.

The carrier gas was helium at flow rate of 0. Dittrichia viscosa Plant List: The fact that almost all the major compound groups detected on the TLC of the leaf extracts can also be detected in the callus extract and the retention of the cell’s ability to produce secondary metabolites by the second generation of calluses are encouraging factors for the possible industrial production of active compounds from mass-tissue cultures.

View large Download slide. This page was last edited on 22 Marchat Nowadays it is quite common in roadsides and ruderal habitats, even in urban areas. Viscksa do not leach from the vacuole if fixation temperatures are kept low; the cell structures are thus retained and artefacts are missing see nucleus and mitochondria in Figs 12, 13, Co-chromatography of the previous two extracts is shown in the middle.

Unula are spherical in shape always appear rounded in sections and of lipid nature as evidence by the fact that they are stained dark with Sudan Black B Fig.

Inula viscosa Honey

In other projects Wikimedia Commons Wikispecies. A similar study was carried out simultaneously on the secretory activity of two generations of calluses produced from leaf tissue.

Abstract Leaves of Inula viscosa have been used in medicine from ancient times. Wikispecies has information related to Dittrichia viscosa. Active compound extraction would permit conservation of the species in the wild because raw material will originate from the laboratory.

Some of the reactions are also presented in Figures 4— Application of GC-MS to callus extracts traced only hydrocarbons and compounds of lipid nature, all of them of minor importance, whereas the volatile compounds present in the leaf extracts were not detected.


Spongy parenchyma is rather compact, leaving only a few intercellular spaces Figs 6, 8, Histochemical analyses of laticifers and glandular trichomes in Cannabis sativa. Pollination of an endangered Caladenia species Orchidaceae by nectar-foraging behaviour of a widespread species of colletid bee. The negative response to DMB staining for phenolic tannin precursors, in both leaves and calluses, although tannins are present in large amounts as vacuolar inclusions in most leaf cells, indicates that the chemical constituents of the condensed tannins are produced prematurely in the leaf cells and condensation proceeds promptly at an early stage of leaf development.

A glycosyl analogue of diacylglycerol and other antiinflammatory constituents from Inula viscosa. Glandular trichomes of Calceolaria adscendens Lidl. Wikimedia Commons has media related to Dittrichia viscosa. Semi-thin sections of plastic embedded tissue pet were stained with: A new histochemical method for localization of the site of monoterpene phenol accumulation in plant secretory structures.

Even though these reactions could not be localized in certain cellular components of callus cells, it is important that non-differentiated cells retained their ability to synthesize and accumulate secondary metabolites of pharmaceutical value terpenes, steroids, alkaloids.

Increased chloroplast activity is indicated by the large starch granules accumulated within them Figs 13, Calluses formed prior to yeast infection develop a zone of inhibition against contaminating yeasts indicating the production and release of anti-fungal factors from undifferentiated cultured cells.

Effect of Inula viscosa extract on chitin synthesis unula dermatophytes and Candida albicans.

Histochemical investigation of leaves. Three cells of the latter, the terminal cell being much longer than the other two and pointed, comprise the long part of the hair Fig. All histochemical reagents used for the leaf viwcosa also applied to the cultured cells. For these reasons, this species is of major interest for many pharmaceutical industries and a detailed study of its secreting structures is necessary.

Original light micrographs were recorded: Flavonoids, the compounds responsible for the antioxidant effects of I.