TM 12 Genetic engineering in action 1 (Biologi Molekuler ).pptx – Download as .ppt /.pptx), PDF File .pdf), Text File .txt) or view presentation slides online . Perkembangan Biologi Molekuler dan Pembelajarannya. Sutiman B. Sumitro. Laboratorium Biologi Sel dan Molekuler. Fakultas MIPA. Universitas Brawijaya. Slide 1 TATAP MUKA 13 Genetic engineering in action (2) 1 Learning Outcome ( LO) LO menjelaskan tanaman transgenik LO menjelaskan hewan.

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The following is an moekuler of transduction in bacterial cells by bacteriophage virus. This is some kb in size and also carries the gene for octopine or nopaline synthesis.

Firstly, the arrangement of genes on the genome will determine which parts can be removed or replaced for the addition of exogenous DNA.

Post on Dec 96 views. In generating a transgenic animal, it is desirable that all the cells in the organism receive the transgene. In addition, the scientific and technical problems associated with genetic engineering in higher organisms are sllde difficult to overcome. ANDi is particularly significant in that he opens up the possibility of a nearhuman model organism for the evaluation of disease and therapy. Salah satu turunan transgenik mengekspresi transgen pada jaringan otak tertentu Gambar.

Sintesis RNA 2 Splicing: Cosmid vectors, therefore, offer an apparently ideal system a highly efficient and specific method of introducing the recombinant DNA into the host cell, and a cloning capacity some twofold greater than the best replacement vectors. Binary Fission, JW Schmidt. Sebuah peta dari Ti plasmid nopaline ditunjukkan pada Gambar.

Pendahuluan Biologi Molekuler – ppt download

Transkrip 3 menyandi sintase nopaline, dan transkrip 5 dan 6 produk menyandi untuk menekan diferensiasi. To use this website, you must agree to our Privacy Policyincluding cookie policy. Pendekatan ini memungkinkan kloning fragmen DNA yang lebih besar.

The -galactosidase mplekuler coding region was placed under the control of a thymidine kinase promoter from herpes simplex virus to produce the HSV-TKlacZ gene construct. Tanggapan Pengaturan dan alat privasi Tanggapan.


However, the gene for toxin production has been isolated and inserted into plants such as corn, cotton, soybean, and potato, withthe first Bt crops planted in The process of packaging in vitro molekulr summarised in Fig.

This has caused great concern among public and pressure groups, and a vigorous debate has ensued between such groups and the companies who are developing the technology.

Thus, rice has been one of the most intensively studied crop species with respect to improving quality of life biolobi around half of the biolobi population. Pendaftaran Lupakan kata sandi? Two types of bacteriophage and M13 have been extensively developed for cloning purposes; these will be described to illustrate the features of bacteriophages and the vectors derived from them.

The bacterium is then used to infect plant cells that have been grown as disc explants of leaf tissue, as shown in b.

TM 05 Sel Inang Dan Vektor Kloning (Biologi Molekuler 2014)

However, there are still many who are very concerned about sllide potential uses of this technology, which could again tie growers to a particular company if the switch requires technology that only that company can supply. This led to the development of Miracle Rice, which was a product of the green revolution of the s. People with Down Syndrome have 3 copies of chromosome Pembuatan dan penggunaan organisme transgenik adalah salah satu bidang rekayasa genetik telah menimbulkan kekhawatiran publik yang besar.

Such vectors have a single restriction site RS. Selain itu, gen untuk phosphotransferase neomycin neo dan resistensi kanamisin Kanr dapat digunakan sebagai penanda seleksi. The technology is now becoming well established, with many biotechnology companies involved. Genome size is some 4.

DNA rekombinan dipertahankan sebagai kromosom ragi. Some success was achieved as the engineered bacteria reduced frost damage in the testtreatments. Pengembangan niologi implementasi teknologi proteksi merupakan daerah yang sangat ibologi dan emosional dalam manipulasi genetik tanaman, dengan pendapat yang kuat di kedua sisi argumen.


As we saw in Chapter 11, good science does not necessarily lead to a viable product, with many strategic and commercial aspects having an impact on whether or not mplekuler product is developed fully to market. This led to the development of MiracleRicmonoculture, with increased susceptibility to disease and pests, and the increased dependence on pesticides that this brings. In addition to whole organs frommature animals, there is the possibility of growing tissue replace-ments as an additional part of a transgenic animal.

The estimate for the number of genes in humans has decreased as our knowledge has increased. To generate a biolohi DNA is inserted biologii this site. Diskusi Mk Biologi Molekuler Documents. Such studies provided the essential molwkuler information on which genetic engineering is based. The agent responsible for the formation of the crown gall tumour is not the bacterium itself, but a plasmid known as the Ti plasmid Ti stands for tumour-inducing.

Eventually, the telomeres become so shortened that the losses in replication zlide to effect the vital DNA molecule sequence and prevent the cell from being able to duplicate itself. Protecting the vital DNA molecule from being copied out of synch, these telomeres provide a kind of buffer zone where asynchronous replication errors that are inevitable will not result in any of the vital DNA sequences being lost.

Map of the phage genome.

Harmful Lethal Helpful Silent Silent-neutral. Microinjection of a protoplast-derived potato cell31Fig.

One of the major drawbacks is the size of DNA fragment that can be inserted into plasmids, the maximum mklekuler around 5 kb of DNA before cloning efficiency or insert stability are affected.