BGI 7003 PDF

results EN ISO ) Module Vof 14 Technical solutions Example: reduction of the dissipation [ ] BGI Evaluation of the. A median number of 7, to 8, expressed genes were detected per cell ( Additional file 4: Supplementary Fig. S4d), including TFs that were. ; 7(10): – .. We wish to acknowledge the help of the BGI- Shenzhen for sequencing and Biochain-Beijing for array CGH.

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However, the corresponding underlying mechanisms of the regulation of cell fate commitment during early neural differentiation remain largely unknown. 70003 dot represents an individual cell.

Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm. These results showed widespread and comprehensive chromatin structure reprogramming during neural differentiation.

To validate the finding and to explain the origin of this sub-chromosomal CNV, we collected fetal bi fluid and parental blood samples and tested the samples using array-based comparative genomic hybridization aCGH. We observed that the number of gained peaks was with the largest increase at the NPCs stage, while the number of lost peaks was relatively high at Ros-E stage Additional file 2: By integrating single cell-based transcriptome profiling of cells from five differentiation stages, we identified a variety of TFs that were differentially expressed throughout the differentiation process and showed distinct expression profiles among specific cell stages.

In the present study, we observed two normal karyotype cases that both vgi the same microdeletion detected by NIPT. Moreover, the results were uniformed across these cell lines Fig. Notch signaling regulates the differentiation of neural crest from human pluripotent stem cells.

Mutation of prdm1 in zebrafish resulted in severe phenotypes with a decrease in the quantity of neural crest cells and the reduction in the size of structures derived from the neural crest [ 57 ].

Global variation in copy number in the human genome. Noninvasive prenatal testing Five milliliters of maternal peripheral blood was collected into a blood collection tube containing ethylenediaminetetraacetic acid dipotassium salt EDTA-K2and the maternal plasma was separated and transferred into a new tube after centrifuging the sample at g for 10 min.

To reveal the detail of chromatin accessibility dynamics during neural differentiation, we also analyzed the gained or lost peaks at each stage compared with the previously neighboring one. In the present study, we used scRNA-seq and assay for transposase-accessible chromatin using sequencing ATAC-seq to investigate human early neural differentiation. Further, communication network analysis demonstrated that cellular interactions most frequently occurred in the embryoid body stage and that each cell subpopulation possessed a distinctive spectrum of ligands and receptors associated with neural differentiation that could reflect the identity of each subpopulation.


Different transcription factors regulate nestin gene expression during P19 cell neural differentiation and central nervous system development.

Genetic effects of a 13q microdeletion detected by noninvasive prenatal testing (NIPT)

The human fibroblast cell line was derived from the dermal skin of a healthy donor, with written informed consent. This study indicated that this microdeletion could be considered as a normal polymorphism at the chromosomal level. To obtain more detailed information regarding this microdeletion, further studies are required.

At last, single cells remained for further analysis in this study. This article has been cited by other articles in PMC. Validation of neural differentiation in different genetic background cell lines.

There were no significant aberrations found in the paternal blood examination. Next, we studied the genomic distribution of these dynamic peaks and gbi that both the gained and lost peaks were located mostly in distal intergenic regions and promoter regions Additional file 2: Zebrafish narrowminded disrupts the transcription factor prdm1 and is required for neural crest and sensory neuron specification.

To study the genomic features associated with 7003 chromatin regions, we classified ATAC peaks based on the location of the peak center. Email alerts New issue alert.

In the case of iPSCs, all peaks were annotated as novel peaks. Based on FCAPS analysis of maternal plasma, the t-score of the chromosomal section adjacent to position 80 Mb was significantly lower than the rest of chromosome 13, indicating a suspicious 3.

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Distinct requirements for wnt9a and irf6 in extension and integration mechanisms during zebrafish palate morphogenesis.

Reference component analysis of single-cell transcriptomes elucidates cellular heterogeneity in human colorectal tumors. The variable genes were selected using the Seurat R package [ 86 ]. Conversely, prdm1 is dispensable for neural crest formation in mice and, instead, is required for primordial germ cell specification, suggesting that the neural crest specification function of prdm1 in mice has been lost [ 71 ]. In addition, we dissected the dynamics of chromatin accessibility at the neural differentiation stages and revealed active cis -regulatory elements for transcription factors known to have a key role in neural differentiation as well as for those that we suggest are also involved.


Single-cell RNA-seq reveals dynamic transcriptome profiling in human early neural differentiation Zhouchun Shang. We analyzed several differentiation stages of cells, including hiPSCs, embryoid body EBearly rosettes hereafter termed Ros-E, post-3 days of rosette formationlate rosettes hereafter termed Ros-L, post-5 days of rosette formationNPCs, and the original somatic fibroblasts Fib.

Novel peaks were defined as peaks nonoverlapping with previous stages. Consistent with these previous studies, in our in vitro system, treatment with SB, in combination with dorsomorphin, results in a dramatic decrease in NANOG expression and a concomitant increase in PAX6 expression Fig. After delivery, the normal phenotype of the newborn infant confirmed our prediction. We also captured bulk transcriptome profiles of the corresponding neural differentiation stages derived from iPSCs and ESCs for validation.

Computational analysis of cell-to-cell heterogeneity in single-cell RNA-sequencing data reveals hidden subpopulations of cells. Microdeletions of chromosome 13q The endothelin receptor-B is required for the migration of neural crest-derived melanocyte and enteric neuron precursors. Stage-specific peaks were defined as peaks having no overlap with any peaks in other stages. Abstract Microdeletions of chromosome 13q Noninvasive prenatal diagnosis of fetal chromosomal aneuploidy by massively parallel genomic sequencing of DNA in maternal plasma.

In contrast, much fewer interactions were predicted after the EB stage, suggesting communications decreased dramatically during the progression of lineage commitment.

A possible resolution for the above problem is to capture the sample with much more precise time points, which may, to some extent, overcome this limitation. First, we reconstructed the differentiation trajectory using 8, genes with variable expression. NIPT was initially used in the prediction of T13, T18, T21 and sex chromosome abnormalities in prenatal diagnosis studies.

Detection of microdeletion 22q Receive exclusive offers and updates from Oxford Academic. Molecular diversity of midbrain development in mouse, human, and stem cells. Genome scan for Tourette disorder in affected-sibling-pair and multigenerational families. To avoid invasive prenatal diagnosis procedures, an NIPT was offered to further screen for common fetal chromosomal abnormalities. S5bwe proposed the Fib2 subset might possess high potential for iPSCs reprogramming and neural conversion.